Novel Single-Molecule Technique by Single-Object Scattering Sampling (SOSS)

Prospect of Retrieving Vibrational Wave Function by Single-Object Scattering Sampling.

The exact shape of wave functions has never been directly measured because an ensemble measurement is often overwhelmed by the contributions of highly populated configurations. In this work, we explore the possibility of directly obtaining vibrational wave functions by single-object scattering sampling (SOSS) using intense, ultrashort X-ray pulses provided by X-ray free electron lasers. Previou...

Novel fluorophores for single-molecule imaging.

Nonlinear optical chromophores based on dicyanodihydrofuran acceptors paired with amine donors have been found to exhibit sufficiently large fluorescence quantum yields and stability to enable single-molecule detection in polymeric hosts. To illustrate the breadth of this class, six fluorophores are presented, spanning the emission range from 505 to 646 nm. In contrast to conventional single-mo...

Single-Molecule Single-Nanoparticle Microscopy

Based on a scanning near-field optical microscope (SNOM) we present measurements on the optical interaction of a single molecule and a single gold nanoparticle. The phenomena of field enhancement, emission quenching and fluorescence enhancement are studied with outstanding control over all key parameters. We have attached a single gold nanoparticle to the extremity of a pulled glass fiber tip a...

Bayesian orientation estimate from single molecule x-ray scattering data

Incoherent x-ray scattering in single molecule imaging

Imaging of the structure of single proteins or other biomolecules with atomic resolution would be enormously beneficial to structural biology. X-ray freeelectron lasers generate highly intense and ultrashort x-ray pulses, providing a route towards imaging of single molecules with atomic resolution. The information on molecular structure is encoded in the coherent x-ray scattering signal. In con...